Human NME3 Recombinant Protein (N-His) (HC575012)

Name: Human NME3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC575012
Price: 211 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) :

Spécification :

50ug
100ug
1mg

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue	HC575012
Description	Recombinant Human NME3 Protein, N-His (HC575012) expressed in E. coli, spanning Met1-Tyr168. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q13232
Longueur de protéine	Met1-Tyr168
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	21.05 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	NDPKC, NME3, DR-nm23, Nucleoside diphosphate kinase C, NDP kinase 3, Nucleoside diphosphate kinase 3, NDK 3, nm23-H3
Contexte	Nucleoside diphosphate kinase C (NME3) is a ~19 kDa protein. Catalyzes the transfer of a gamma-phosphoryl group from a nucleoside triphosphate, mainly ATP, to a nucleoside diphosphate via a ping-pong mechanism involving a phosphohistidine intermediate, therefore contributing to the nucleoside triphosphate homeostasis. In vitro, can also use other phosphate donors such as UTP and GTP. Independently of its nucleoside diphosphate kinase activity, involved in mitochondrial membrane tethering, a prerequisite for fusion through direct membrane-binding and hexamerization. Involved in DNA repair of both single- and double-stranded breaks by associating with the ribonucleotide reductase (RNR) complex via interaction with the histone acetyltransferase KAT5, facilitating recruitment to DNA damage sites independently of its kinase activity. Inhibits granulocyte differentiation. 1. Erent, M. et al. (2001) European journal of biochemistry 268, 1972-81. PMID: 11277919 2. Chen, CW. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 566-574. PMID: 30587587 3. Amjadi, R. et al. (2024) International journal of molecular sciences 25. PMID: 39337255 4. Su, YA. et al. (2023) The Journal of cell biology 222. PMID: 37584589 5. Tsao, N. et al. (2016) The Biochemical journal 473, 1237-45. PMID: 26945015 6. Venturelli, D. et al. (1995) Proceedings of the National Academy of Sciences of the United States of America 92, 7435-9. PMID: 7638209
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human NME3 Protein, N-His

Reférences

Fiche technique

Human NME3 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai