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| カタログ番号 | HF013022 |
|---|---|
| 説明 |
Recombinant Human NPPA Protein, N-His-KSI (HF013022) expressed in E. coli, spanning Asn26-Tyr151. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P01160 |
| タンパク質長 | Asn26-Tyr151 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.44 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Natriuretic peptides A, Atrial natriuretic factor prohormone, proANF, Atrial natriuretic peptide prohormone, preproANP, proANP, Atriopeptigen, Cardiodilatin, CDD, preproCDD-ANF, Long-acting natriuretic peptide, LANP, Long-acting natriuretic hormone, LANH, Pro atrial natriuretic factor 1-30, proANF 1-30, Pro atrial natriuretic peptide 1-30, proANP 1-30, Vessel dilator, VSDL, Pro atrial natriuretic factor 31-67, proANF 31-67, Pro atrial natriuretic peptide 31-67, proANP 31-67, Kaliuretic peptide, KP, Pro atrial natriuretic factor 79-98, proANF 79-98, Pro atrial natriuretic peptide 79-98, proANP 79-98, Urodilatin, URO, CDD 95-126, CDD-ANP (95-126), Pro atrial natriuretic peptide 95-126, proANP 95-126, Auriculin-C, Atrial natriuretic factor 1-33, ANF 1-33, Auriculin-D, Atrial natriuretic factor 3-33, ANF 3-33, Atrial natriuretic peptide, ANP, Alpha-atrial natriuretic peptide, Alpha-hANP, Atrial natriuretic factor, ANF, CDD-ANF, CDD-ANP (99-126), Cardionatrin, Pro atrial natriuretic factor 99-126, proANF 99-126, Auriculin-B, Atrial natriuretic factor 8-33, ANF 8-33, Auriculin-A, Atriopeptin-1, Atriopeptin I, Atriopeptin-2, Atriopeptin II, Atriopeptin-3, Atriopeptin III, NPPA |
| 背景 | Natriuretic peptides A is a ~16 kDa protein. Hormone that plays a key role in mediating cardio-renal homeostasis, and is involved in vascular remodeling and regulating energy metabolism. Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins, such as PRKG1, that drive various biological responses. Regulates vasodilation, natriuresis, diuresis and aldosterone synthesis and is therefore essential for regulating blood pressure, controlling the extracellular fluid volume and maintaining the fluid-electrolyte balance. Also involved in inhibiting cardiac remodeling and cardiac hypertrophy by inducing cardiomyocyte apoptosis and attenuating the growth of cardiomyocytes and fibroblasts. Plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus, and thus prevents pregnancy-induced hypertension. 1. Birkenfeld, AL. et al. (2005) The Journal of clinical endocrinology and metabolism 90, 3622-8. PMID: 15741263 2. Rubattu, S. et al. (2006) Journal of the American College of Cardiology 48, 499-505. PMID: 16875975 3. Birkenfeld, AL. et al. (2008) Diabetes 57, 3199-204. PMID: 18835931 4. Souza, SC. et al. (2011) Biochemical and biophysical research communications 410, 398-403. PMID: 21672517 5. Bordicchia, M. et al. (2012) The Journal of clinical investigation 122, 1022-36. PMID: 22307324 6. Vesely, DL. et al. (1989) Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.) 192, 230-5. PMID: 2532366 7. Vesely, DL. et al. (1987) Biochemical and biophysical research communications 148, 1540-8. PMID: 2825692 8. Vesely, DL. et al. (1995) The Journal of endocrinology 146, 373-80. PMID: 7595132 9. Chiou, S. et al. (1995) Endocrinology 136, 2033-9. PMID: 7720651 10. Vesely, DL. et al. (1994) Circulation 90, 1129-40. PMID: 8087923 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human NPPA Protein, N-His-KSI
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