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| カタログ番号 | HD550012 |
|---|---|
| 説明 |
Recombinant Human NPRL3 Protein, N-His (HD550012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q12980 |
| タンパク質長 | Met1-Pro569 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | -14 gene protein, Alpha-globin regulatory element-containing gene protein, C16orf35, CGTHBA, GATOR1 complex protein NPRL3, MARE, NPRL3, Nitrogen permease regulator 3-like protein, Protein CGTHBA |
| 背景 | GATOR1 complex protein NPRL3 is a ~63 kDa protein. As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the mTORC1 pathway. In response to amino acid depletion, the GATOR1 complex has GTPase activating protein (GAP) activity and strongly increases GTP hydrolysis by RagA/RRAGA (or RagB/RRAGB) within heterodimeric Rag complexes, thereby turning them into their inactive GDP-bound form, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. In the presence of abundant amino acids, the GATOR1 complex is negatively regulated by GATOR2, the other GATOR subcomplex, in this amino acid-sensing branch of the TORC1 pathway. 1. Bar-Peled, L. et al. (2013) Science (New York, N.Y.) 340, 1100-6. PMID: 23723238 2. Shen, K. et al. (2018) Nature 556, 64-69. PMID: 29590090 3. Egri, SB. et al. (2022) Molecular cell 82, 1836-1849.e5. PMID: 35338845 |
| 注意事項 | For research use only |
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