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Обзор
Каталожный номерHC721012
Описание
Recombinant Human NUF2 Protein, N-His (HC721012) expressed in E. coli, spanning Met1-Ser171. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессииE. coli
Номер доступаQ9BZD4
Длина белкаMet1-Ser171
ПримененияELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
ВидыHomo sapiens (Human)
Характер Recombinant
Уровень эндотоксинов Please contact with the lab for this information.
Чистота >90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса 22.24 kDa
Форма Lyophilized
Буфер хранения Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Реконституция Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Доставка In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названияCDCA1, Cell division cycle-associated protein 1, Kinetochore protein Nuf2, NUF2, NUF2R, hNuf2, hNuf2R, hsNuf2
Фон

Kinetochore protein Nuf2 is a ~54 kDa protein. Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

1. DeLuca, JG. et al. (2002) The Journal of cell biology 159, 549-55. PMID: 12438418
2. DeLuca, JG. et al. (2003) Current biology : CB 13, 2103-9. PMID: 14654001
3. Joseph, J. et al. (2004) Current biology : CB 14, 611-7. PMID: 15062103
4. Stucke, VM. et al. (2004) Chromosoma 113, 1-15. PMID: 15235793
5. Meraldi, P. et al. (2004) Developmental cell 7, 45-60. PMID: 15239953
6. DeLuca, JG. et al. (2005) Molecular biology of the cell 16, 519-31. PMID: 15548592
7. Liu, D. et al. (2007) The Journal of biological chemistry 282, 21415-24. PMID: 17535814
8. Schmidt, JC. et al. (2012) Developmental cell 23, 968-80. PMID: 23085020
Примечание For research use only
Изображения
Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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