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| カタログ番号 | HC722012 |
|---|---|
| 説明 |
Recombinant Human ORAI3 Protein, N-His-SUMO (HC722012) expressed in E. coli, spanning Gly178-Gln243. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9BRQ5 |
| タンパク質長 | Gly178-Gln243 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 18.68 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ORAI3, Protein orai-3, TMEM142C, Transmembrane protein 142C |
| 背景 | Protein orai-3 is a ~31 kDa protein. Pore-forming subunit of two major inward rectifying Ca(2+) channels at the plasma membrane: Ca(2+) release-activated Ca(2+) (CRAC) channels and arachidonate-regulated Ca(2+)-selective (ARC) channels. Assembles with ORAI1 and ORAI2 to form hexameric CRAC channels that mediate Ca(2+) influx upon depletion of endoplasmic reticulum Ca(2+) store and channel activation by Ca(2+) sensor STIM1, a process known as store-operated Ca(2+) entry (SOCE). Various pore subunit combinations may account for distinct CRAC channel spatiotemporal and cell-type specific dynamics. ORAI1 mainly contributes to the generation of Ca(2+) plateaus involved in sustained Ca(2+) entry and is dispensable for cytosolic Ca(2+) oscillations, whereas ORAI2 and ORAI3 generate oscillatory patterns. CRAC channels assemble in Ca(2+) signaling microdomains where Ca(2+) influx is coupled to calmodulin and calcineurin signaling and activation of NFAT transcription factors recruited to ORAI1 via AKAP5. 1. Mercer, JC. et al. (2006) The Journal of biological chemistry 281, 24979-90. PMID: 16807233 2. Lis, A. et al. (2007) Current biology : CB 17, 794-800. PMID: 17442569 3. Yuan, JP. et al. (2009) Nature cell biology 11, 337-43. PMID: 19182790 4. Mignen, O. et al. (2009) The Journal of physiology 587, 4181-97. PMID: 19622606 5. Lee, KP. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 14687-92. PMID: 19706554 6. Bogeski, I. et al. (2010) Science signaling 3, ra24. PMID: 20354224 7. Yoast, RE. et al. (2020) Nature communications 11, 2444. PMID: 32415068 |
| 注意事項 | For research use only |
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