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| カタログ番号 | HC195012 |
|---|---|
| 説明 |
Recombinant Human OSBP Protein, N-His (HC195012) expressed in E. coli, spanning Ser416-Lys654. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P22059 |
| タンパク質長 | Ser416-Lys654 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 29.63 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | OSBP, OSBP1, Oxysterol-binding protein 1 |
| 背景 | Oxysterol-binding protein 1 is a ~89 kDa protein. Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol (cholesterol) with phosphatidylinositol 4-phosphate (PI4P, 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 4-phosphate)), delivering sterol to the Golgi in exchange for PI4P, which is subsequently degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing the stability of the relatively short lived ABCA1 protein (an important point of control for cholesterol efflux activity). 1. Mesmin, B. et al. (2013) Cell 155, 830-43. PMID: 24209621 2. Mesmin, B. et al. (2017) The EMBO journal 36, 3156-3174. PMID: 28978670 3. Wang, PY. et al. (2005) Science (New York, N.Y.) 307, 1472-6. PMID: 15746430 4. Suchanek, M. et al. (2007) The Biochemical journal 405, 473-80. PMID: 17428193 5. Bowden, K. et al. (2008) The Journal of biological chemistry 283, 18210-7. PMID: 18450749 |
| 注意事項 | For research use only |
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