Human PARG Recombinant Protein (N-His) (HV273012)

Name: Human PARG Recombinant Protein (N-His)
Brand: abinScience
SKU: HV273012
Price: 211 USD
Availability: InStock

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Número de catálogo	HV273012
Descripción	Recombinant Human PARG Protein, N-His (HV273012) expressed in E. coli, spanning Ser448-Thr976. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q86W56
Longitud de proteína	Ser448-Thr976
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	63.36 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	PARG, Poly(ADP-ribose) glycohydrolase
Fondo	Poly(ADP-ribose) glycohydrolase (PARG) is a ~111 kDa protein. Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose). Mainly acts as an exo-glycohydrolase but can act as an endo-glycohydrolase at low efficiency, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers. It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated. Poly(ADP-ribose) synthesized after DNA damage is only present transiently and is rapidly degraded by PARG. Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress. 1. Putt, KS. et al. (2004) Analytical biochemistry 333, 256-64. PMID: 15450800 2. Slade, D. et al. (2011) Nature 477, 616-20. PMID: 21892188 3. Le May, N. et al. (2012) Molecular cell 48, 785-98. PMID: 23102699 4. Rosenthal, F. et al. (2013) Nature structural & molecular biology 20, 502-7. PMID: 23474714 5. Bonfiglio, JJ. et al. (2020) Cell 183, 1086-1102.e23. PMID: 33186521 6. Prokhorova, E. et al. (2021) Molecular cell 81, 2640-2655.e8. PMID: 34019811 7. Rack, JGM. et al. (2021) Nature communications 12, 4581. PMID: 34321462 8. Sharifi, R. et al. (2013) The EMBO journal 32, 1225-37. PMID: 23481255 9. Fontana, P. et al. (2023) Nature communications 14, 3200. PMID: 37268618
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Human PARG Recombinant Protein (N-His)

Referencias

Datasheet

Human PARG Recombinant Protein (N-His).pdf

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