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| カタログ番号 | HV249022 |
|---|---|
| 説明 |
Recombinant Human PARP2 Protein, N-GST & C-His (HV249022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9UGN5 |
| タンパク質長 | Ser223-Trp583 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ADP-ribosyltransferase diphtheria toxin-like 2, ADPRT-2, ADPRT2, ADPRTL2, ARTD2, DNA ADP-ribosyltransferase PARP2, EC:2.4.2.-, EC:2.4.2.30, NAD(+) ADP-ribosyltransferase 2, PARP-2, PARP2, Poly [ADP-ribose] polymerase 2, Poly[ADP-ribose] synthase 2, Protein poly-ADP-ribosyltransferase PARP2, hPARP-2, pADPRT-2 |
| 背景 | Poly [ADP-ribose] polymerase 2 is a ~66 kDa protein. Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair. Mediates glutamate, aspartate or serine ADP-ribosylation of proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1. Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site. 1. Amé, JC. et al. (1999) The Journal of biological chemistry 274, 17860-8. PMID: 10364231 2. Vyas, S. et al. (2014) Nature communications 5, 4426. PMID: 25043379 3. Talhaoui, I. et al. (2016) Nucleic acids research 44, 9279-9295. PMID: 27471034 4. Chen, Q. et al. (2018) Nature communications 9, 3233. PMID: 30104678 5. Suskiewicz, MJ. et al. (2020) Nature 579, 598-602. PMID: 32028527 6. Bilokapic, S. et al. (2020) Nature 585, 609-613. PMID: 32939087 7. Obaji, E. et al. (2021) Nature communications 12, 3479. PMID: 34108479 8. Bacic, L. et al. (2021) eLife 10. PMID: 34486521 9. Mohapatra, J. et al. (2021) eLife 10. PMID: 34874266 |
| 注意事項 | For research use only |
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