中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HD908012 |
|---|---|
| 説明 |
Recombinant Human PARP9 Protein, N-His (HD908012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8IXQ6 |
| タンパク質長 | Asn310-Asn493 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | ADP-ribosyltransferase diphtheria toxin-like 9, ARTD9, B aggressive lymphoma protein, BAL, BAL1, EC:2.4.2.-, PARP-9, PARP9, Poly [ADP-ribose] polymerase 9, Protein mono-ADP-ribosyltransferase PARP9 |
| 背景 | Protein mono-ADP-ribosyltransferase PARP9 is a ~96 kDa protein. ADP-ribosyltransferase which, in association with E3 ligase DTX3L, plays a role in DNA damage repair and in immune responses including interferon-mediated antiviral defenses. Within the complex, enhances DTX3L E3 ligase activity which is further enhanced by PARP9 binding to poly(ADP-ribose). In association with DTX3L and in presence of E1 and E2 enzymes, mediates NAD(+)-dependent mono-ADP-ribosylation of ubiquitin which prevents ubiquitin conjugation to substrates such as histones. During DNA repair, PARP1 recruits PARP9/BAL1-DTX3L complex to DNA damage sites via PARP9 binding to ribosylated PARP1. Subsequent PARP1-dependent PARP9/BAL1-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. 1. Juszczynski, P. et al. (2006) Molecular and cellular biology 26, 5348-59. PMID: 16809771 2. Yan, Q. et al. (2013) Molecular and cellular biology 33, 845-57. PMID: 23230272 3. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788 4. Iwata, H. et al. (2016) Nature communications 7, 12849. PMID: 27796300 5. Yang, CS. et al. (2017) Molecular cell 66, 503-516.e5. PMID: 28525742 |
| 注意事項 | For research use only |
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
