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| カタログ番号 | HC900012 |
|---|---|
| 説明 |
Recombinant Human PHF1 Protein, N-His (HC900012) expressed in E. coli, spanning Leu31-Arg334. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O43189 |
| タンパク質長 | Leu31-Arg334 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.90 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | PCL1, PHD finger protein 1, PHF1, Polycomb-like protein 1, Protein PHF1, hPCl1, hPHF1 |
| 背景 | PHD finger protein 1 is a ~62 kDa protein. Polycomb group (PcG) that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex. Involved in DNA damage response and is recruited at double-strand breaks (DSBs). Acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting the PRC2 complex: it is however unclear whether recruitment of the PRC2 complex to H3K36me3 leads to enhance or inhibit H3K27me3 methylation mediated by the PRC2 complex. According to some reports, PRC2 recruitment by PHF1 promotes H3K27me3 and subsequent gene silencing by inducing spreading of PRC2 and H3K27me3 into H3K36me3 loci. According to another report, PHF1 recruits the PRC2 complex at double-strand breaks (DSBs) and inhibits the activity of PRC2. 1. Sarma, K. et al. (2008) Molecular and cellular biology 28, 2718-31. PMID: 18285464 2. Cai, L. et al. (2013) Molecular cell 49, 571-82. PMID: 23273982 3. Musselman, CA. et al. (2012) Nature structural & molecular biology 19, 1266-72. PMID: 23142980 |
| 注意事項 | For research use only |
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