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| カタログ番号 | HC221012 |
|---|---|
| 説明 |
Recombinant Human PHF19 Protein, N-His (HC221012) expressed in E. coli, spanning Asn22-Lys350. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q5T6S3 |
| タンパク質長 | Asn22-Lys350 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 40.20 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | PCL3, PHD finger protein 19, PHF19, Polycomb-like protein 3, hPCL3 |
| 背景 | PHD finger protein 19 is a ~65 kDa protein. Polycomb group (PcG) protein that specifically binds histone H3 trimethylated at 'Lys-36' (H3K36me3) and recruits the PRC2 complex, thus enhancing PRC2 H3K27me3 methylation activity. Probably involved in the transition from an active state to a repressed state in embryonic stem cells: acts by binding to H3K36me3, a mark for transcriptional activation, and recruiting H3K36me3 histone demethylases RIOX1 or KDM2B, leading to demethylation of H3K36 and recruitment of the PRC2 complex that mediates H3K27me3 methylation, followed by de novo silencing. Recruits the PRC2 complex to CpG islands and contributes to embryonic stem cell self-renewal. Also binds histone H3 dimethylated at 'Lys-36' (H3K36me2). Isoform 1 and isoform 2 inhibit transcription from an HSV-tk promoter. 1. Wang, S. et al. (2004) Gene 343, 69-78. PMID: 15563832 2. Daub, H. et al. (2008) Molecular cell 31, 438-48. PMID: 18691976 3. Ballaré, C. et al. (2012) Nature structural & molecular biology 19, 1257-65. PMID: 23104054 4. Brien, GL. et al. (2012) Nature structural & molecular biology 19, 1273-81. PMID: 23160351 5. Qin, S. et al. (2013) Biochemical and biophysical research communications 430, 547-53. PMID: 23228662 6. Cai, L. et al. (2013) Molecular cell 49, 571-82. PMID: 23273982 7. Chen, S. et al. (2018) Molecular cell 69, 840-852.e5. PMID: 29499137 8. Chen, S. et al. (2020) Molecular cell 77, 1265-1278.e7. PMID: 31959557 |
| 注意事項 | For research use only |
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