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| カタログ番号 | HC963012 |
|---|---|
| 説明 |
Recombinant Human PIAS1 Protein, N-His (HC963012) expressed in E. coli, spanning Gln139-Lys420. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O75925 |
| タンパク質長 | Gln139-Lys420 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 34.15 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DDXBP1, DEAD/H box-binding protein 1, E3 SUMO-protein ligase PIAS1, E3 SUMO-protein transferase PIAS1, EC:2.3.2.-, GBP, Gu-binding protein, PIAS1, Protein inhibitor of activated STAT protein 1, RNA helicase II-binding protein |
| 背景 | E3 SUMO-protein ligase PIAS1 is a ~71 kDa protein. Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Catalyzes sumoylation of various proteins, such as CEBPB, MRE11, MTA1, PTK2, PML and ZNF76. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. In vitro, binds A/T-rich DNA. The effects of this transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context. 1. Kahyo, T. et al. (2001) Molecular cell 8, 713-8. PMID: 11583632 2. Schmidt, D. et al. (2002) Proceedings of the National Academy of Sciences of the United States of America 99, 2872-7. PMID: 11867732 3. Kadaré, G. et al. (2003) The Journal of biological chemistry 278, 47434-40. PMID: 14500712 4. Zheng, G. et al. (2004) The Journal of biological chemistry 279, 42410-21. PMID: 15280358 5. Cong, L. et al. (2011) The Journal of biological chemistry 286, 43793-43808. PMID: 21965678 6. Zhang, T. et al. (2022) Nature communications 13, 5133. PMID: 36050397 |
| 注意事項 | For research use only |
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