Human PIEZO1 Recombinant Protein (N-His) (HP708012)

Name: Human PIEZO1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP708012
Price: 211 USD
Availability: InStock

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Número de catálogo	HP708012
Descripción	Recombinant Human PIEZO1 Protein, N-His (HP708012) expressed in E. coli, spanning Gln2206-Lys2426. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q92508
Longitud de proteína	Gln2206-Lys2426
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	27.11 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Piezo-type mechanosensitive ion channel component 1, FAM38A, PIEZO1, Protein FAM38A, KIAA0233, Mib, Membrane protein induced by beta-amyloid treatment
Fondo	Piezo-type mechanosensitive ion channel component 1 (PIEZO1) is a ~286 kDa protein. Pore-forming subunit of the mechanosensitive non-specific cation Piezo channel required for rapidly adapting mechanically activated (MA) currents and has a key role in sensing touch and tactile pain. Piezo channels are homotrimeric three-blade propeller-shaped structures that utilize a cap-motion and plug-and-latch mechanism to gate their ion-conducting pathways. Generates currents characterized by a linear current-voltage relationship that are sensitive to ruthenium red and gadolinium. Conductance to monovalent alkali ions is highest for K(+), intermediate for Na(+) and lowest for Li(+). Divalent ions except for Mn(2+) permeate the channel but more slowly than the monovalent ions and they also reduce K(+) currents. 1. Andolfo, I. et al. (2013) Blood 121, 3925-35, S1-12. PMID: 23479567 2. Albuisson, J. et al. (2013) Nature communications 4, 1884. PMID: 23695678 3. Gnanasambandam, R. et al. (2015) PloS one 10, e0125503. PMID: 25955826 4. Zhou, Z. et al. (2023) Science (New York, N.Y.) 381, 799-804. PMID: 37590348 5. McHugh, BJ. et al. (2010) Journal of cell science 123, 51-61. PMID: 20016066 6. Li, J. et al. (2014) Nature 515, 279-282. PMID: 25119035 7. Tsuchiya, M. et al. (2018) Nature communications 9, 2049. PMID: 29799007
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human PIEZO1 Protein, N-His

Referencias

Datasheet

Human PIEZO1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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