Human PIK3C3 Recombinant Protein (N-His) (HV616012)

Name: Human PIK3C3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV616012
Price: 211 USD
Availability: InStock

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Vista general

Número de catálogo	HV616012
Descripción	Recombinant Human PIK3C3 Protein, N-His (HV616012) expressed in E. coli, spanning Tyr631-Trp885. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q8NEB9
Longitud de proteína	Tyr631-Trp885
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	31.5 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	PtdIns-3-kinase type 3, Phosphatidylinositol 3-kinase p100 subunit, hVps34, Phosphatidylinositol 3-kinase catalytic subunit type 3, Phosphoinositide-3-kinase class 3, PI3-kinase type 3, VPS34, PI3K type 3, PIK3C3
Fondo	Phosphatidylinositol 3-kinase catalytic subunit type 3 (PIK3C3) is a ~101 kDa protein. Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. As part of PI3KC3-C1, promotes endoplasmic reticulum membrane curvature formation prior to vesicle budding. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes. 1. Stein, MP. et al. (2003) Traffic (Copenhagen, Denmark) 4, 754-71. PMID: 14617358 2. Chen, YH. et al. (2021) Nature communications 12, 1322. PMID: 33637724 3. Volinia, S. et al. (1995) The EMBO journal 14, 3339-48. PMID: 7628435 4. Zhang, BC. et al. (2020) Nature immunology 21, 868-879. PMID: 32690950 5. Sagona, AP. et al. (2010) Nature cell biology 12, 362-71. PMID: 20208530 6. Thoresen, SB. et al. (2010) Experimental cell research 316, 3368-78. PMID: 20643123
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human PIK3C3 Protein, N-His

Referencias

Datasheet

Human PIK3C3 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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