Human PIN1 Recombinant Protein (N-GST) (HC505032)

Name: Human PIN1 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HC505032
Price: 211 USD
Availability: InStock

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100ug
1mg

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SDS

Vista general

Número de catálogo	HC505032
Descripción	Recombinant Human PIN1 Protein, N-GST (HC505032) expressed in E. coli, spanning Glu51-Glu163. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q13526
Longitud de proteína	Glu51-Glu163
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	39.51 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Rotamase Pin1, Peptidyl-prolyl cis-trans isomerase Pin1, PPIase Pin1, PIN1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
Fondo	Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 (PIN1) is a ~18 kDa protein. Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs. By inducing conformational changes in a subset of phosphorylated proteins, acts as a molecular switch in multiple cellular processes. Displays a preference for acidic residues located N-terminally to the proline bond to be isomerized. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. 1. Lee, TH. et al. (2011) Molecular cell 42, 147-59. PMID: 21497122 2. Steger, M. et al. (2013) Molecular cell 50, 333-43. PMID: 23623683 3. Nechama, M. et al. (2018) Nature communications 9, 1603. PMID: 29686383 4. Lim, JH. et al. (2011) The Journal of biological chemistry 286, 44403-11. PMID: 22033920 5. Yu, L. et al. (2006) The Journal of biological chemistry 281, 18201-7. PMID: 16644721 6. Dougherty, MK. et al. (2005) Molecular cell 17, 215-24. PMID: 15664191 7. Phan, RT. et al. (2007) Nature immunology 8, 1132-9. PMID: 17828269 8. Min, SH. et al. (2012) Molecular cell 46, 771-83. PMID: 22608923 10. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human PIN1 Protein, N-GST

Referencias

Datasheet

Human PIN1 Recombinant Protein (N-GST).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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