Human PKP1 Recombinant Protein (N-GST) (HC672012)

Name: Human PKP1 Recombinant Protein (N-GST)
Brand: abinScience
SKU: HC672012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HC672012
Descripción	Recombinant Human PKP1 Protein, N-GST (HC672012) expressed in E. coli, spanning Asn500-Asn742. Purity: >90% by SDS-PAGE. Highlights ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q13835
Longitud de proteína	Asn500-Asn742
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	53.25 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	B6P, Plakophilin-1, Band 6 protein, PKP1
Fondo	Plakophilin-1 (PKP1) is a ~82 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Plays a role in desmosome protein expression regulation and localization to the desmosomal plaque, thereby maintaining cell sheet integrity and anchorage of desmosomes to intermediate filaments. Required for localization of DSG3 and YAP1 to the cell membrane in keratinocytes in response to mechanical strain, via the formation of an interaction complex composed of DSG3, YAP1, PKP1 and YWHAG. Positively regulates differentiation of keratinocytes, potentially via promoting localization of DSG1 at desmosome cell junctions. Required for calcium-independent development and maturation of desmosome plaques specifically at lateral cell-cell contacts in differentiating keratinocytes. 1. Wolf, A. et al. (2013) Journal of cell science 126, 1832-44. PMID: 23444369 2. Hofmann, I. et al. (2000) Journal of cell science 113 ( Pt 13), 2471-83. PMID: 10852826 3. Uttagomol, J. et al. (2019) International journal of molecular sciences 20. PMID: 31835537 4. McGrath, JA. et al. (1997) Nature genetics 17, 240-4. PMID: 9326952 5. Sobolik-Delmaire, T. et al. (2010) The Journal of investigative dermatology 130, 2638-46. PMID: 20613778 6. Wolf, A. et al. (2010) The Journal of cell biology 188, 463-71. PMID: 20156963 7. Fischer-Kešo, R. et al. (2014) Molecular and cellular biology 34, 4244-56. PMID: 25225333
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human PKP1 Protein, N-GST

Referencias

Datasheet

Human PKP1 Recombinant Protein (N-GST).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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