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| カタログ番号 | HV058012 |
|---|---|
| 説明 |
Recombinant Human PKP2 Protein, N-His (HV058012) expressed in E. coli, spanning Asp571-Ala849. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q99959 |
| タンパク質長 | Asp571-Ala849 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 25.22 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | PKP2, Plakophilin-2 |
| 背景 | Plakophilin-2 (PKP2) is a ~97 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins. Required to maintain gingival epithelial barrier function. Important component of the desmosome that is also required for localization of desmosome component proteins such as DSC2, DSG2 and JUP to the desmosome cell-cell junction. Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls. 1. Todorovic, V. et al. (2014) Molecular biology of the cell 25, 3749-64. PMID: 25208567 2. Koetsier, JL. et al. (2014) The Journal of investigative dermatology 134, 112-122. PMID: 23884246 3. Yu, N. et al. (2021) Journal of periodontal research 56, 1046-1057. PMID: 34368962 4. Kirchner, F. et al. (2012) Circulation. Cardiovascular genetics 5, 400-11. PMID: 22781308 5. Pérez-Hernández, M. et al. (2022) Circulation 146, 851-867. PMID: 35959657 6. Sobolik-Delmaire, T. et al. (2010) The Journal of investigative dermatology 130, 2638-46. PMID: 20613778 7. Fidler, LM. et al. (2009) Journal of cellular and molecular medicine 13, 4219-28. PMID: 18662195 8. Wang, L. et al. (2017) Nature communications 8, 13876. PMID: 28169297 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PKP2 Protein, N-His
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