Human PKP2 Recombinant Protein (N-His) (HV058012)

Name: Human PKP2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV058012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HV058012
Описание	Recombinant Human PKP2 Protein, N-His (HV058012) expressed in E. coli, spanning Asp571-Ala849. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q99959
Длина белка	Asp571-Ala849
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	25.22 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	PKP2, Plakophilin-2
Фон	Plakophilin-2 (PKP2) is a ~97 kDa protein. A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion. Regulates focal adhesion turnover resulting in changes in focal adhesion size, cell adhesion and cell spreading, potentially via transcriptional modulation of beta-integrins. Required to maintain gingival epithelial barrier function. Important component of the desmosome that is also required for localization of desmosome component proteins such as DSC2, DSG2 and JUP to the desmosome cell-cell junction. Required for the formation of desmosome cell junctions in cardiomyocytes, thereby required for the correct formation of the heart, specifically trabeculation and formation of the atria walls. 1. Todorovic, V. et al. (2014) Molecular biology of the cell 25, 3749-64. PMID: 25208567 2. Koetsier, JL. et al. (2014) The Journal of investigative dermatology 134, 112-122. PMID: 23884246 3. Yu, N. et al. (2021) Journal of periodontal research 56, 1046-1057. PMID: 34368962 4. Kirchner, F. et al. (2012) Circulation. Cardiovascular genetics 5, 400-11. PMID: 22781308 5. Pérez-Hernández, M. et al. (2022) Circulation 146, 851-867. PMID: 35959657 6. Sobolik-Delmaire, T. et al. (2010) The Journal of investigative dermatology 130, 2638-46. PMID: 20613778 7. Fidler, LM. et al. (2009) Journal of cellular and molecular medicine 13, 4219-28. PMID: 18662195 8. Wang, L. et al. (2017) Nature communications 8, 13876. PMID: 28169297
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human PKP2 Protein, N-His

Ссылки

Даташит

Human PKP2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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