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| カタログ番号 | HC725012 |
|---|---|
| 説明 |
Recombinant Human PLA2G4E Protein, N-His (HC725012) expressed in E. coli, spanning Ser60-Glu327. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q3MJ16 |
| タンパク質長 | Ser60-Glu327 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 32.98 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Calcium-dependent N-acyltransferase, Cytosolic phospholipase A2 epsilon, EC:3.1.1.4, PLA2G4E, Phospholipase A2 group IVE, cPLA2-epsilon |
| 背景 | Cytosolic phospholipase A2 epsilon is a ~99 kDa protein. Calcium-dependent N-acyltransferase involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain. Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide. Has weak phospholipase A2 and lysophospholipase activities. 1. Hussain, Z. et al. (2018) Biochimica et biophysica acta. Molecular and cell biology of lipids 1863, 493-502. PMID: 29447909 2. Binte Mustafiz, SS. et al. (2019) Journal of biochemistry 165, 343-352. PMID: 30517655 |
| 注意事項 | For research use only |
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