Human PLOD3 Recombinant Protein (N-His) (HT299012)

Name: Human PLOD3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT299012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HT299012
Description	Recombinant Human PLOD3 Protein, N-His (HT299012) expressed in E. coli, spanning Asn35-Asn255. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O60568
Longueur de protéine	Asn35-Asn255
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	27.75 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	LH3, Lysyl hydroxylase 3, Procollagen galactosyltransferase, Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3, Galactosylhydroxylysine-glucosyltransferase, PLOD3, Procollagen glucosyltransferase
Contexte	Multifunctional procollagen lysine hydroxylase and glycosyltransferase LH3 (PLOD3) is a ~84 kDa protein. Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine. Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. 1. Rautavuoma, K. et al. (2002) The Journal of biological chemistry 277, 23084-91. PMID: 11956192 2. Wang, C. et al. (2002) Matrix biology : journal of the International Society for Matrix Biology 21, 559-66. PMID: 12475640 3. Wang, C. et al. (2009) Journal of cellular and molecular medicine 13, 508-21. PMID: 18298658 4. Salo, AM. et al. (2008) American journal of human genetics 83, 495-503. PMID: 18834968 5. Scietti, L. et al. (2018) Nature communications 9, 3163. PMID: 30089812
Note	For research use only.

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SDS-PAGE for Recombinant Human PLOD3 Protein, N-His

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Human PLOD3 Recombinant Protein (N-His).pdf

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Formula

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