Human POLD2 Recombinant Protein (N-His) (HA706012)

Name: Human POLD2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA706012
Price: 211 USD
Availability: InStock

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100ug
1mg

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SDS

Vista general

Número de catálogo	HA706012
Descripción	Recombinant Human POLD2 Protein, N-His (HA706012) expressed in E. coli, spanning Met1-Pro469. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P49005
Longitud de proteína	Met1-Pro469
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	53.45 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	DNA polymerase delta subunit 2; DNA polymerase delta subunit p50; POLD2
Fondo	DNA polymerase delta subunit 2 is a ~51 kDa protein. Accessory component of both the DNA polymerase delta complex and the DNA polymerase zeta complex. As a component of the trimeric and tetrameric DNA polymerase delta complexes (Pol-delta3 and Pol-delta4, respectively), plays a role in high fidelity genome replication, including in lagging strand synthesis, and repair. Pol-delta3 and Pol-delta4 are characterized by the absence or the presence of POLD4. They exhibit differences in catalytic activity. Most notably, Pol-delta3 shows higher proofreading activity than Pol-delta4. 1. Zhang, S. et al. (2007) The Journal of biological chemistry 282, 15330-40. PMID: 17317665 2. Chea, J. et al. (2012) Cell cycle (Georgetown, Tex.) 11, 2885-95. PMID: 22801543 3. Lee, YS. et al. (2014) Proceedings of the National Academy of Sciences of the United States of America 111, 2954-9. PMID: 24449906 4. Xie, B. et al. (2002) Biochemistry 41, 13133-42. PMID: 12403614 5. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 6. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 7. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 8. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 9. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374 10. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611
Nota	For research use only

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human POLD2 Protein, N-His

Referencias

Datasheet

Human POLD2 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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