Human POLQ Recombinant Protein (N-His) (HF853021)

Name: Human POLQ Recombinant Protein (N-His)
Brand: abinScience
SKU: HF853021
Price: 264 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo	HF853021
Descripción	Recombinant Human POLQ Protein, N-His (HF853021) expressed in Insect Cells, spanning Ser1819-Val2590. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	Insect cells
Número de acceso	O75417
Longitud de proteína	Ser1819-Val2590
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	89.00 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	POLH, DNA polymerase eta, DNA polymerase theta, POLQ
Fondo	DNA polymerase theta (POLQ) is a ~289 kDa protein. Low-fidelity DNA polymerase with a helicase activity that promotes microhomology-mediated end-joining (MMEJ), an alternative non-homologous end-joining (NHEJ) machinery required to repair double-strand breaks in DNA during mitosis. MMEJ is an error-prone repair pathway that produces deletions of sequences from the strand being repaired and promotes genomic rearrangements, such as telomere fusions, some of them leading to cellular transformation. MMEJ is required during mitosis to repair persistent double-strand breaks that originate in S-phase. Although error-prone, MMEJ protects against chromosomal instability and tumorigenesis. The polymerase acts by binding directly the 2 ends of resected double-strand breaks, allowing microhomologous sequences in the overhangs to form base pairs. 1. Seki, M. et al. (2003) Nucleic acids research 31, 6117-26. PMID: 14576298 2. Arana, ME. et al. (2008) Nucleic acids research 36, 3847-56. PMID: 18503084 3. Yoon, JH. et al. (2014) The Journal of biological chemistry 289, 13177-85. PMID: 24648516 4. Ceccaldi, R. et al. (2015) Nature 518, 258-62. PMID: 25642963 5. Kent, T. et al. (2015) Nature structural & molecular biology 22, 230-7. PMID: 25643323 6. Zahn, KE. et al. (2015) Nature structural & molecular biology 22, 304-11. PMID: 25775267 7. Newman, JA. et al. (2015) Structure (London, England : 1993) 23, 2319-2330. PMID: 26636256 8. Kent, T. et al. (2016) eLife 5. PMID: 27311885 9. Kent, T. et al. (2016) Nucleic acids research 44, 9381-9392. PMID: 27591252 10. Wang, Z. et al. (2019) The Journal of biological chemistry 294, 3909-3919. PMID: 30655289
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human POLQ Protein, N-His

Referencias

Datasheet

Human POLQ Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo