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| カタログ番号 | HT290012 |
|---|---|
| 説明 |
Recombinant Human POLR3G Protein, N-His (HT290012) expressed in E. coli, spanning Met1-Met110. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O15318 |
| タンパク質長 | Met1-Met110 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.27 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DNA-directed RNA polymerase III subunit RPC7, RNA polymerase III subunit C7, DNA-directed RNA polymerase III subunit G, RNA polymerase III 32 kDa apha subunit, RPC32-alpha, RNA polymerase III 32 kDa subunit, RPC32, POLR3G |
| 背景 | DNA-directed RNA polymerase III subunit RPC7 (POLR3G) is a ~25 kDa protein. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III (Pol III) which synthesizes small non-coding RNAs including 5S rRNA, snRNAs, tRNAs and miRNAs from at least 500 distinct genomic loci. Acts as a long tether that bridges POLR3C/RPC3-POLR3F/RPC6-POLR3G/RPC7 heterotrimer and the mobile stalk of Pol III, coordinating the dynamics of Pol III stalk and clamp modules during the transition from apo to elongation state. Pol III exists as two alternative complexes defined by the mutually exclusive incorporation of subunit POLR3G/RPC7alpha or POLR3GL/RPC7beta. POLR3G/RPC7alpha modulates Pol III transcriptome by specifically enhancing the transcription of snaR-A non-coding RNAs. 1. Canella, D. et al. (2010) Genome research 20, 710-21. PMID: 20413673 2. Girbig, M. et al. (2021) Nature structural & molecular biology 28, 210-219. PMID: 33558764 3. Hou, H. et al. (2021) Nature communications 12, 6135. PMID: 34675218 4. Van Bortle, K. et al. (2022) Nature communications 13, 3007. PMID: 35637192 5. Haurie, V. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 4176-81. PMID: 20154270 6. Wang, Q. et al. (2021) Nature structural & molecular biology 28, 220-227. PMID: 33558766 7. Ablasser, A. et al. (2009) Nature immunology 10, 1065-72. PMID: 19609254 8. Chiu, YH. et al. (2009) Cell 138, 576-91. PMID: 19631370 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human POLR3G Protein, N-His
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