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Human POU2F2 Recombinant Protein (N-His) (HY048012)

Human POU2F2 Recombinant Protein (N-His)
Human POU2F2 Recombinant Protein (N-His)
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  • データシート

  • SDS

概要
カタログ番号HY048012
説明
Recombinant Human POU2F2 Protein, N-His (HY048012) expressed in E. coli, spanning Phe236-Thr398. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●E. coli Expression — High-yield, cost-effective production.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムE. coli
アクセッション番号P09086
タンパク質長Phe236-Thr398
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 20.41 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.

RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名Oct-2, POU domain, class 2, transcription factor 2, OTF-2, OCT2, Octamer-binding protein 2, POU2F2, Lymphoid-restricted immunoglobulin octamer-binding protein NF-A2, Octamer-binding transcription factor 2, OTF2
背景

POU domain, class 2, transcription factor 2 (POU2F2) is a ~51 kDa protein. Transcription factor that specifically binds to the octamer motif (5'-ATTTGCAT-3'). Regulates IL6 expression in B cells with POU2AF1. Regulates transcription in a number of tissues in addition to activating immunoglobulin gene expression. Modulates transcription transactivation by NR3C1, AR and PGR.

1. Scheidereit, C. et al. (1988) Nature 336, 551-7. PMID: 2904654
2. Strubin, M. et al. (1995) Cell 80, 497-506. PMID: 7859290
3. Ko, HS. et al. (1988) Cell 55, 135-44. PMID: 2901913
4. Préfontaine, GG. et al. (1999) The Journal of biological chemistry 274, 26713-9. PMID: 10480874
注意事項 For research use only.
イメージ
  • Human POU2F2 Recombinant Protein (N-His)

    SDS-PAGE

    SDS-PAGE for Recombinant Human POU2F2 Protein, N-His

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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