Human PPP2R1A Recombinant Protein (N-His) (HB105012)

Name: Human PPP2R1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB105012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HB105012
Описание	Recombinant Human PPP2R1A Protein, N-His (HB105012) expressed in E. coli, spanning Met1-Ala589. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P30153
Длина белка	Met1-Ala589
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	67.47 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, Medium tumor antigen-associated 61 kDa protein, PP2A subunit A isoform PR65-alpha, PP2A subunit A isoform R1-alpha, PPP2R1A
Фон	Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform (PPP2R1A) is a ~65 kDa protein. The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis. Together with RACK1 adapter, mediates dephosphorylation of AKT1 at 'Ser-473', preventing AKT1 activation and AKT-mTOR signaling pathway. Dephosphorylation of AKT1 is essential for regulatory T-cells (Treg) homeostasis and stability. 1. Zhu, D. et al. (2004) The Journal of biological chemistry 279, 54983-6. PMID: 15525651 2. Tang, Z. et al. (2006) Developmental cell 10, 575-85. PMID: 16580887 3. Zheng, H. et al. (2020) Science (New York, N.Y.) 370. PMID: 33243860 4. Jeong, BC. et al. (2021) Nature structural & molecular biology 28, 290-299. PMID: 33633399 5. Vervoort, SJ. et al. (2021) Cell 184, 3143-3162.e32. PMID: 34004147 6. Zolnierowicz, S. et al. (1996) The Biochemical journal 317 ( Pt 1), 187-94. PMID: 8694763 7. Goudreault, M. et al. (2009) Molecular & cellular proteomics : MCP 8, 157-71. PMID: 18782753 8. Sivakumar, S. et al. (2017) Biology open 6, 1672-1679. PMID: 28982702
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human PPP2R1A Protein, N-His

Ссылки

Даташит

Human PPP2R1A Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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