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| カタログ番号 | HW456012 |
|---|---|
| 説明 |
Recombinant Human PPP5C Protein, N-His (HW456012) expressed in E. coli, spanning Asp22-Met499. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P53041 |
| タンパク質長 | Asp22-Met499 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 56.90 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | PP-T, Protein phosphatase T, Serine/threonine-protein phosphatase 5, PPP5, PPT, PP5, PPP5C |
| 背景 | Serine/threonine-protein phosphatase 5 (PPP5C) is a ~56 kDa protein. Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. 1. Wechsler, T. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 1247-52. PMID: 14734805 2. Ikeda, K. et al. (2004) Molecular endocrinology (Baltimore, Md.) 18, 1131-43. PMID: 14764652 3. Ali, A. et al. (2004) Genes & development 18, 249-54. PMID: 14871926 4. Zeke, T. et al. (2005) The Biochemical journal 385, 45-56. PMID: 15383005 5. Liu, F. et al. (2005) The Journal of biological chemistry 280, 1790-6. PMID: 15546861 6. Zhang, J. et al. (2005) Molecular and cellular biology 25, 9910-9. PMID: 16260606 7. Partch, CL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 10467-10472. PMID: 16790549 8. von Kriegsheim, A. et al. (2006) Nature cell biology 8, 1011-6. PMID: 16892053 9. Kang, Y. et al. (2009) The Journal of biological chemistry 284, 9845-53. PMID: 19176521 10. Chatterjee, A. et al. (2010) The Journal of biological chemistry 285, 3872-3882. PMID: 19948726 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PPP5C Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
