Human PPP5C Recombinant Protein (N-His) (HW456012)

Name: Human PPP5C Recombinant Protein (N-His)
Brand: abinScience
SKU: HW456012
Price: 211 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug
1mg

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HW456012
Описание	Recombinant Human PPP5C Protein, N-His (HW456012) expressed in E. coli, spanning Asp22-Met499. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P53041
Длина белка	Asp22-Met499
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	56.90 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	PP-T, Protein phosphatase T, Serine/threonine-protein phosphatase 5, PPP5, PPT, PP5, PPP5C
Фон	Serine/threonine-protein phosphatase 5 (PPP5C) is a ~56 kDa protein. Serine/threonine-protein phosphatase that dephosphorylates a myriad of proteins involved in different signaling pathways including the kinases CSNK1E, ASK1/MAP3K5, PRKDC and RAF1, the nuclear receptors NR3C1, PPARG, ESR1 and ESR2, SMAD proteins and TAU/MAPT. Implicated in wide ranging cellular processes, including apoptosis, differentiation, DNA damage response, cell survival, regulation of ion channels or circadian rhythms, in response to steroid and thyroid hormones, calcium, fatty acids, TGF-beta as well as oxidative and genotoxic stresses. Participates in the control of DNA damage response mechanisms such as checkpoint activation and DNA damage repair through, for instance, the regulation ATM/ATR-signaling and dephosphorylation of PRKDC and TP53BP1. Inhibits ASK1/MAP3K5-mediated apoptosis induced by oxidative stress. Plays a positive role in adipogenesis, mainly through the dephosphorylation and activation of PPARG transactivation function. 1. Wechsler, T. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 1247-52. PMID: 14734805 2. Ikeda, K. et al. (2004) Molecular endocrinology (Baltimore, Md.) 18, 1131-43. PMID: 14764652 3. Ali, A. et al. (2004) Genes & development 18, 249-54. PMID: 14871926 4. Zeke, T. et al. (2005) The Biochemical journal 385, 45-56. PMID: 15383005 5. Liu, F. et al. (2005) The Journal of biological chemistry 280, 1790-6. PMID: 15546861 6. Zhang, J. et al. (2005) Molecular and cellular biology 25, 9910-9. PMID: 16260606 7. Partch, CL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 10467-10472. PMID: 16790549 8. von Kriegsheim, A. et al. (2006) Nature cell biology 8, 1011-6. PMID: 16892053 9. Kang, Y. et al. (2009) The Journal of biological chemistry 284, 9845-53. PMID: 19176521 10. Chatterjee, A. et al. (2010) The Journal of biological chemistry 285, 3872-3882. PMID: 19948726
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human PPP5C Protein, N-His

Ссылки

Даташит

Human PPP5C Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа