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| カタログ番号 | HD935012 |
|---|---|
| 説明 |
Recombinant Human PRKDC Protein, N-His (HD935012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P78527 |
| タンパク質長 | Arg1816-Pro2202 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DNA-PK catalytic subunit, DNA-PKcs, DNA-dependent protein kinase catalytic subunit, DNPK1, EC:2.7.11.1, HYRC, HYRC1, PRKDC, S473K, Ser-473 kinase, p460 |
| 背景 | DNA-dependent protein kinase catalytic subunit is a ~469 kDa protein. Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). Recruited by XRCC5 and XRCC6 to DNA ends and is required to (1) protect and align broken ends of DNA, thereby preventing their degradation, (2) and sequester the DSB for repair by NHEJ. 1. Ma, Y. et al. (2002) Cell 108, 781-94. PMID: 11955432 2. Soubeyrand, S. et al. (2003) Cancer research 63, 1198-201. PMID: 12649176 3. Wechsler, T. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 1247-52. PMID: 14734805 4. Chen, S. et al. (2021) Nature 593, 294-298. PMID: 33854234 5. Chaplin, AK. et al. (2021) Molecular cell 81, 3400-3409.e3. PMID: 34352203 |
| 注意事項 | For research use only |
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