Human PRKN Recombinant Protein (N-His) (HT315012)

Name: Human PRKN Recombinant Protein (N-His)
Brand: abinScience
SKU: HT315012
Price: 211 USD
Availability: InStock

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Número de catálogo	HT315012
Descripción	Recombinant Human PRKN Protein, N-His (HT315012) expressed in E. coli, spanning Ile229-Val465. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	O60260
Longitud de proteína	Ile229-Val465
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	28.99 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Parkin, PARK2, Parkinson juvenile disease protein 2, E3 ubiquitin-protein ligase parkin, Parkin RBR E3 ubiquitin-protein ligase, PRKN, Parkinson disease protein 2
Fondo	E3 ubiquitin-protein ligase parkin (PRKN) is a ~51 kDa protein. Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. Substrates include SYT11 and VDAC1. Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2. Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context. Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation. 1. Shimura, H. et al. (2000) Nature genetics 25, 302-5. PMID: 10888878 2. Imai, Y. et al. (2000) The Journal of biological chemistry 275, 35661-4. PMID: 10973942 3. Shimura, H. et al. (2001) Science (New York, N.Y.) 293, 263-9. PMID: 11431533 4. Imai, Y. et al. (2002) Molecular cell 10, 55-67. PMID: 12150907 5. Staropoli, JF. et al. (2003) Neuron 37, 735-49. PMID: 12628165 6. Chung, KK. et al. (2004) Science (New York, N.Y.) 304, 1328-31. PMID: 15105460 7. Ko, HS. et al. (2005) The Journal of neuroscience : the official journal of the Society for Neuroscience 25, 7968-78. PMID: 16135753 8. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232 9. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592 10. Liu, S. et al. (2012) PLoS genetics 8, e1002537. PMID: 22396657
Nota	For research use only.

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SDS-PAGE

SDS-PAGE for Human PRKN Recombinant Protein (N-His)

Referencias

Datasheet

Human PRKN Recombinant Protein (N-His).pdf

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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