Human PRMT1 Recombinant Protein (N-His) (HA119012)

Name: Human PRMT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA119012
Price: 211 USD
Availability: InStock

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Número de catálogo	HA119012
Descripción	Recombinant Human PRMT1 Protein, N-His (HA119012) expressed in E. coli, spanning Gly61-Arg371. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q99873
Longitud de proteína	Gly61-Arg371
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	38.25 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	IR1B4, Protein arginine N-methyltransferase 1, HRMT1L2, HMT2, Interferon receptor 1-bound protein 4, PRMT1, Histone-arginine N-methyltransferase PRMT1
Fondo	Protein arginine N-methyltransferase 1 (PRMT1) is a ~42 kDa protein. Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-3' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates MRE11 and TP53BP1, promoting the DNA damage response. 1. Tang, J. et al. (2000) The Journal of biological chemistry 275, 19866-76. PMID: 10749851 2. Boisvert, FM. et al. (2005) Genes & development 19, 671-6. PMID: 15741314 3. Passos, DO. et al. (2006) The FEBS journal 273, 3946-61. PMID: 16879614 4. Yamagata, K. et al. (2008) Molecular cell 32, 221-31. PMID: 18951090 5. Cho, JH. et al. (2012) Cell death and differentiation 19, 859-70. PMID: 22095282 6. Takai, H. et al. (2014) Cell reports 9, 48-60. PMID: 25284789 7. Zhang, L. et al. (2015) eLife 4. PMID: 26575292 8. Toma-Fukai, S. et al. (2016) Journal of molecular biology 428, 1197-1208. PMID: 26876602 9. Madreiter-Sokolowski, CT. et al. (2016) Nature communications 7, 12897. PMID: 27642082 10. Tsang, B. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4218-4227. PMID: 30765518
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Human PRMT1 Recombinant Protein (N-His)

Referencias

Datasheet

Human PRMT1 Recombinant Protein (N-His).pdf

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Formula

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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