Human PRMT1 Recombinant Protein (N-His) (HA119012)

Name: Human PRMT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HA119012
Price: 211 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD):

Спецификация:

50ug
100ug
1mg

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер	HA119012
Описание	Recombinant Human PRMT1 Protein, N-His (HA119012) expressed in E. coli, spanning Gly61-Arg371. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q99873
Длина белка	Gly61-Arg371
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	38.25 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	IR1B4, Protein arginine N-methyltransferase 1, HRMT1L2, HMT2, Interferon receptor 1-bound protein 4, PRMT1, Histone-arginine N-methyltransferase PRMT1
Фон	Protein arginine N-methyltransferase 1 (PRMT1) is a ~42 kDa protein. Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, FMR1, ILF3, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15, EWS, HABP4, SERBP1, RBM15, FOXO1, CHTOP, MAP3K5/ASK1, MICU1 and NPRL2. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-3' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Methylates RBM15, promoting ubiquitination and degradation of RBM15. Methylates MRE11 and TP53BP1, promoting the DNA damage response. 1. Tang, J. et al. (2000) The Journal of biological chemistry 275, 19866-76. PMID: 10749851 2. Boisvert, FM. et al. (2005) Genes & development 19, 671-6. PMID: 15741314 3. Passos, DO. et al. (2006) The FEBS journal 273, 3946-61. PMID: 16879614 4. Yamagata, K. et al. (2008) Molecular cell 32, 221-31. PMID: 18951090 5. Cho, JH. et al. (2012) Cell death and differentiation 19, 859-70. PMID: 22095282 6. Takai, H. et al. (2014) Cell reports 9, 48-60. PMID: 25284789 7. Zhang, L. et al. (2015) eLife 4. PMID: 26575292 8. Toma-Fukai, S. et al. (2016) Journal of molecular biology 428, 1197-1208. PMID: 26876602 9. Madreiter-Sokolowski, CT. et al. (2016) Nature communications 7, 12897. PMID: 27642082 10. Tsang, B. et al. (2019) Proceedings of the National Academy of Sciences of the United States of America 116, 4218-4227. PMID: 30765518
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Human PRMT1 Recombinant Protein (N-His)

Ссылки

Даташит

Human PRMT1 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа