Human RFWD3 Recombinant Protein (N-His) (HP698012)

Name: Human RFWD3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HP698012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HP698012
Description	Recombinant Human RFWD3 Protein, N-His (HP698012) expressed in E. coli, spanning Gly430-Glu774. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q6PCD5
Longueur de protéine	Gly430-Glu774
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	40.51 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	RING finger and WD repeat domain-containing protein 3, RNF201, E3 ubiquitin-protein ligase RFWD3, RING finger protein 201, RFWD3
Contexte	E3 ubiquitin-protein ligase RFWD3 is a ~85 kDa protein. E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage. Plays a key role in RPA-mediated DNA damage signaling and repair. Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination. Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint. May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. 1. Gong, Z. et al. (2011) The Journal of biological chemistry 286, 22308-13. PMID: 21504906 2. Liu, S. et al. (2011) The Journal of biological chemistry 286, 22314-22. PMID: 21558276 3. Elia, AE. et al. (2015) Molecular cell 60, 280-93. PMID: 26474068 4. Feeney, L. et al. (2017) Molecular cell 66, 610-621.e4. PMID: 28575657 5. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658 6. Gallina, I. et al. (2021) Molecular cell 81, 442-458.e9. PMID: 33321094
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human RFWD3 Protein, N-His

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Human RFWD3 Recombinant Protein (N-His).pdf

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Formula

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