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| カタログ番号 | HC317022 |
|---|---|
| 説明 |
Recombinant Human RIPK1 Protein, N-GST & C-His (HC317022) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q13546 |
| タンパク質長 | Gln2-Glu295 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Cell death protein RIP, EC:2.7.11.1, RIP, RIP-1, RIP1, RIPK1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1 |
| 背景 | Receptor-interacting serine/threonine-protein kinase 1 is a ~75 kDa protein. Serine-threonine kinase which is a key regulator of TNF-mediated apoptosis, necroptosis and inflammatory pathways. Exhibits kinase activity-dependent functions that regulate cell death and kinase-independent scaffold functions regulating inflammatory signaling and cell survival. Has kinase-independent scaffold functions: upon binding of TNF to TNFR1, RIPK1 is recruited to the TNF-R1 signaling complex (TNF-RSC also known as complex I) where it acts as a scaffold protein promoting cell survival, in part, by activating the canonical NF-kappa-B pathway. Kinase activity is essential to regulate necroptosis and apoptosis, two parallel forms of cell death: upon activation of its protein kinase activity, regulates assembly of two death-inducing complexes, namely complex IIa (RIPK1-FADD-CASP8), which drives apoptosis, and the complex IIb (RIPK1-RIPK3-MLKL), which drives necroptosis. RIPK1 is required to limit CASP8-dependent TNFR1-induced apoptosis. 1. Shembade, N. et al. (2007) The EMBO journal 26, 3910-22. PMID: 17703191 2. Zaman, MM. et al. (2013) Molecular and cellular biology 33, 4971-84. PMID: 24144979 3. Tao, P. et al. (2020) Nature 577, 109-114. PMID: 31827280 4. Lalaoui, N. et al. (2020) Nature 577, 103-108. PMID: 31827281 5. Ashida, H. et al. (2020) The EMBO journal 39, e104469. PMID: 32657447 6. Hou, B. et al. (2022) Cell death discovery 8, 319. PMID: 35831301 7. Holler, N. et al. (2000) Nature immunology 1, 489-95. PMID: 11101870 8. He, S. et al. (2009) Cell 137, 1100-11. PMID: 19524512 9. Cho, YS. et al. (2009) Cell 137, 1112-23. PMID: 19524513 10. Meng, H. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E2001-E2009. PMID: 29440439 |
| 注意事項 | For research use only |
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