中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HT354012 |
|---|---|
| 説明 |
Recombinant Human RIPK2 Protein, N-GST (HT354012) expressed in E. coli, spanning Gln432-Met540. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O43353 |
| タンパク質長 | Gln432-Met540 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 39.28 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Tyrosine-protein kinase RIPK2, CARDIAK, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, RICK, RIPK2, CARD-containing IL-1 beta ICE-kinase, Receptor-interacting serine/threonine-protein kinase 2, RIP-like-interacting CLARP kinase, RIP2, Receptor-interacting protein 2, RIP-2 |
| 背景 | Receptor-interacting serine/threonine-protein kinase 2 (RIPK2) is a ~61 kDa protein. Serine/threonine/tyrosine-protein kinase that plays an essential role in modulation of innate and adaptive immune responses. Acts as a key effector of NOD1 and NOD2 signaling pathways: upon activation by bacterial peptidoglycans, NOD1 and NOD2 oligomerize and recruit RIPK2 via CARD-CARD domains, leading to the formation of RIPK2 filaments. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3, as well as 'Met-1'-linked (linear) polyubiquitination by the LUBAC complex, becoming a scaffolding protein for downstream effectors. 'Met-1'-linked polyubiquitin chains attached to RIPK2 recruit IKBKG/NEMO, which undergoes 'Lys-63'-linked polyubiquitination in a RIPK2-dependent process. 'Lys-63'-linked polyubiquitin chains attached to RIPK2 serve as docking sites for TAB2 and TAB3 and mediate the recruitment of MAP3K7/TAK1 to IKBKG/NEMO, inducing subsequent activation of IKBKB/IKKB. 1. Ruefli-Brasse, AA. et al. (2004) The Journal of biological chemistry 279, 1570-4. PMID: 14638696 2. Manon, F. et al. (2007) Journal of molecular biology 365, 160-74. PMID: 17054981 3. Tigno-Aranjuez, JT. et al. (2010) Genes & development 24, 2666-77. PMID: 21123652 4. Bist, P. et al. (2017) Nature communications 8, 15865. PMID: 28656966 5. Inohara, N. et al. (1998) The Journal of biological chemistry 273, 12296-300. PMID: 9575181 6. McCarthy, JV. et al. (1998) The Journal of biological chemistry 273, 16968-75. PMID: 9642260 7. Abbott, DW. et al. (2007) Molecular and cellular biology 27, 6012-25. PMID: 17562858 8. Damgaard, RB. et al. (2012) Molecular cell 46, 746-58. PMID: 22607974 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RIPK2 Protein, N-GST
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
