Human RIPK3 Recombinant Protein (N-GST & C-His) (HV460022)

Name: Human RIPK3 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HV460022
Price: 211 USD
Availability: InStock

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1mg

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HV460022
説明	Recombinant Human RIPK3 Protein, N-GST & C-His (HV460022) expressed in E. coli, spanning Met1-His132. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
発現システム	E. coli
アクセッション番号	Q9Y572
タンパク質長	Met1-His132
アプリケーション	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種	Homo sapiens (Human)
性質	Recombinant
エンドトキシンレベル	Please contact with the lab for this information.
純度	>90% as determined by SDS-PAGE.
予測分子量	42.96 kDa
形態	Lyophilized
保存バッファー	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
再構成	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名	EC:2.7.11.1, RIP-3, RIP-like protein kinase 3, RIP3, RIPK3, Receptor-interacting protein 3, Receptor-interacting serine/threonine-protein kinase 3
背景	Receptor-interacting serine/threonine-protein kinase 3 is a ~56 kDa protein. Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death. Necroptosis, a programmed cell death process in response to death-inducing TNF family members, is triggered by RIPK3 following activation by ZBP1. Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage. In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol. Also regulates apoptosis: apoptosis depends on RIPK1, FADD and CASP8, and is independent of MLKL and RIPK3 kinase activity. 1. He, S. et al. (2009) Cell 137, 1100-11. PMID: 19524512 2. Cho, YS. et al. (2009) Cell 137, 1112-23. PMID: 19524513 3. Sun, L. et al. (2012) Cell 148, 213-27. PMID: 22265413 4. Wang, Z. et al. (2012) Cell 148, 228-43. PMID: 22265414 5. Zhao, J. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 5322-7. PMID: 22421439 6. Choi, SW. et al. (2018) Molecular cell 70, 920-935.e7. PMID: 29883609 7. Ashida, H. et al. (2020) The EMBO journal 39, e104469. PMID: 32657447 8. Zheng, M. et al. (2020) Cell 181, 674-687.e13. PMID: 32298652
注意事項	For research use only