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Human RNASE6 Recombinant Protein (C-His) (HD339011)

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Aperçu
Numéro de catalogueHD339011
Description
Recombinant Human RNASE6 Protein, C-His (HD339011) expressed in Yeast Cells, spanning Trp24-Leu150. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expressionYeast cells
Numéro d'accèsQ93091
Longueur de protéineTrp24-Leu150
ApplicationsELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspècesHomo sapiens (Human)
Nature Recombinant
Niveau d'endotoxines Please contact with the lab for this information.
Pureté >90% as determined by SDS-PAGE.
Masse moléculaire prédite 15.97 kDa
Forme Lyophilized
Tampon de stockage Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Reconstitution Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Expédition In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifsEC:3.1.27.-, RNASE6, RNS6, RNase K6, Ribonuclease K6
Contexte

Ribonuclease K6 is a ~17 kDa protein. Ribonuclease which shows a preference for the pyrimidines uridine and cytosine. Has potent antibacterial activity against a range of Gram-positive and Gram-negative bacteria, including P.aeruginosa, A.baumanii, M.luteus, S.aureus, E.faecalis, E.faecium, S.saprophyticus and E.coli. Causes loss of bacterial membrane integrity, and also promotes agglutination of Gram-negative bacteria. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.

1. Prats-Ejarque, G. et al. (2016) The Biochemical journal 473, 1523-36. PMID: 27013146
2. Rosenberg, HF. et al. (1996) Nucleic acids research 24, 3507-13. PMID: 8836175
3. Becknell, B. et al. (2015) Kidney international 87, 151-61. PMID: 25075772
4. Pulido, D. et al. (2016) International journal of molecular sciences 17, 552. PMID: 27089320
Note For research use only
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Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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