Human RNF113A Recombinant Protein (N-His) (HB570012)

Name: Human RNF113A Recombinant Protein (N-His)
Brand: abinScience
SKU: HB570012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB570012
Description	Recombinant Human RNF113A Protein, N-His (HB570012) expressed in E. coli, spanning Ile201-Thr343. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O15541
Longueur de protéine	Ile201-Thr343
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	18.77 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	E3 ubiquitin-protein ligase RNF113A; 2.3.2.27; Cwc24 homolog; RING finger protein 113A; Zinc finger protein 183; RNF113A; RNF113; ZNF183
Contexte	E3 ubiquitin-protein ligase RNF113A is a ~38 kDa protein. Required for pre-mRNA splicing as component of the spliceosome. As a component of the minor spliceosome, involved in the splicing of U12-type introns in pre-mRNAs (Probable). E3 ubiquitin-protein ligase that catalyzes the transfer of ubiquitin onto target proteins. Catalyzes polyubiquitination of SNRNP200/BRR2 with non-canonical 'Lys-63'-linked polyubiquitin chains. Plays a role in DNA repair via its role in the synthesis of 'Lys-63'-linked polyubiquitin chains that recruit ALKBH3 and the ASCC complex to sites of DNA damage by alkylating agents. 1. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106 2. Haselbach, D. et al. (2018) Cell 172, 454-464.e11. PMID: 29361316 3. Lear, T. et al. (2017) American journal of physiology. Cell physiology 313, C584-C592. PMID: 28978524 4. Brickner, JR. et al. (2017) Nature 551, 389-393. PMID: 29144457
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human RNF113A Protein, N-His

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Human RNF113A Recombinant Protein (N-His).pdf

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Formula

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