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| カタログ番号 | HB337012 |
|---|---|
| 説明 |
Recombinant Human RNF4 Protein, N-GST & C-His (HB337012) expressed in E. coli, spanning Thr129-Ile190. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P78317 |
| タンパク質長 | Thr129-Ile190 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 35.23 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | E3 ubiquitin-protein ligase RNF4; 2.3.2.27; RING finger protein 4; Small nuclear ring finger protein; Protein SNURF; RNF4; SNURF; RES4-26 |
| 背景 | E3 ubiquitin-protein ligase RNF4 is a ~21 kDa protein. E3 ubiquitin-protein ligase which binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. Regulates the degradation of several proteins including PML and the transcriptional activator PEA3. Involved in chromosome alignment and spindle assembly, it regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. Regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation. 1. Tatham, MH. et al. (2008) Nature cell biology 10, 538-46. PMID: 18408734 2. Guo, B. et al. (2009) Molecular and cellular biology 29, 3204-18. PMID: 19307308 3. Krastev, DB. et al. (2022) Nature cell biology 24, 62-73. PMID: 35013556 4. Geoffroy, MC. et al. (2010) Molecular biology of the cell 21, 4227-39. PMID: 20943951 5. Mukhopadhyay, D. et al. (2010) The Journal of cell biology 188, 681-92. PMID: 20212317 6. Martin, N. et al. (2009) The EMBO journal 28, 3534-48. PMID: 19779455 7. van Hagen, M. et al. (2010) Nucleic acids research 38, 1922-31. PMID: 20026589 8. Kaiser, FJ. et al. (2003) The Journal of biological chemistry 278, 38780-5. PMID: 12885770 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant Human RNF4 Protein, N-GST & C-His
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