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| カタログ番号 | HA686012 |
|---|---|
| 説明 |
Recombinant Human RPA1 Protein, N-His (HA686012) expressed in E. coli, spanning Ile187-Met616. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P27694 |
| タンパク質長 | Ile187-Met616 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 51.04 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Replication protein A 70 kDa DNA-binding subunit; RP-A p70; Replication factor A protein 1; RF-A protein 1; Single-stranded DNA-binding protein; Replication protein A 70 kDa DNA-binding subunit, N-terminally processed; RPA1; REPA1; RPA70 |
| 背景 | Replication protein A 70 kDa DNA-binding subunit is a ~68 kDa protein. As part of the heterotrimeric replication protein A complex (RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage. In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response. 1. Gupta, R. et al. (2007) Blood 110, 2390-8. PMID: 17596542 2. Haahr, P. et al. (2016) Nature cell biology 18, 1196-1207. PMID: 27723717 3. Bass, TE. et al. (2016) Nature cell biology 18, 1185-1195. PMID: 27723720 4. Lin, YL. et al. (1998) The Journal of biological chemistry 273, 1453-61. PMID: 9430682 5. Maréchal, A. et al. (2014) Molecular cell 53, 235-246. PMID: 24332808 6. Sleeth, KM. et al. (2007) Journal of molecular biology 373, 38-47. PMID: 17765923 7. Aboussekhra, A. et al. (1995) Cell 80, 859-68. PMID: 7697716 8. DeMott, MS. et al. (1998) The Journal of biological chemistry 273, 27492-8. PMID: 9765279 9. Grudic, A. et al. (2007) Nucleic acids research 35, 7267-78. PMID: 17959650 10. Sharma, R. et al. (2022) Blood 139, 1039-1051. PMID: 34767620 |
| 注意事項 | For research use only |
SDS-PAGE for Recombinant Human RPA1 Protein, N-His
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