ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
39.07 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
Insulin-stimulated protein kinase 1, MAP kinase-activated protein kinase 1b, ISPK-1, RSK2, Ribosomal S6 kinase 2, MAPKAPK-1b, ISPK1, pp90RSK2, p90RSK3, RSK-2, 90 kDa ribosomal protein S6 kinase 3, MAPKAPK1B, S6K-alpha-3, MAPKAP kinase 1b, p90-RSK 3, RPS6KA3, MAPK-activated protein kinase 1b, Ribosomal protein S6 kinase alpha-3
Фон
Ribosomal protein S6 kinase alpha-3 (RPS6KA3) is a ~83 kDa protein. Serine/threonine-protein kinase that acts downstream of ERK (MAPK1/ERK2 and MAPK3/ERK1) signaling and mediates mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77, regulates translation through RPS6 and EIF4B phosphorylation, and mediates cellular proliferation, survival, and differentiation by modulating mTOR signaling and repressing pro-apoptotic function of BAD and DAPK1. In fibroblast, is required for EGF-stimulated phosphorylation of CREB1 and histone H3 at 'Ser-10', which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF and PMA), phosphorylates and activates NR4A1/NUR77 and ETV1/ER81 transcription factors and the cofactor CREBBP. Upon insulin-derived signal, acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' and inhibiting its activity. Phosphorylates RPS6 in response to serum or EGF via an mTOR-independent mechanism and promotes translation initiation by facilitating assembly of the preinitiation complex.
1. Anjum, R. et al. (2005) Current biology : CB 15, 1762-7. PMID: 16213824
2. Wingate, AD. et al. (2006) The Biochemical journal 393, 715-24. PMID: 16223362
3. Roux, PP. et al. (2007) The Journal of biological chemistry 282, 14056-64. PMID: 17360704
4. De Cesare, D. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 12202-7. PMID: 9770464
5. Sassone-Corsi, P. et al. (1999) Science (New York, N.Y.) 285, 886-91. PMID: 10436156
6. Sutherland, C. et al. (1993) The Biochemical journal 296 ( Pt 1), 15-9. PMID: 8250835
7. Carriere, A. et al. (2008) Frontiers in bioscience : a journal and virtual library 13, 4258-75. PMID: 18508509
9. Carrière, A. et al. (2008) Current biology : CB 18, 1269-77. PMID: 18722121
10. Zhou, Y. et al. (2015) Nature communications 6, 7679. PMID: 26158630
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human RPS6KA3 Protein, N-His
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