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| カタログ番号 | HP575012 |
|---|---|
| 説明 |
Recombinant Human RSAD2 Protein, N-His (HP575012) expressed in E. coli, spanning Ser74-Trp361. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q8WXG1 |
| タンパク質長 | Ser74-Trp361 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 36.02 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | RSAD2, Viperin, Cytomegalovirus-induced gene 5 protein, Radical S-adenosyl methionine domain-containing protein 2, Virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible, CIG5 |
| 背景 | S-adenosylmethionine-dependent nucleotide dehydratase RSAD2 is a ~42 kDa protein. Interferon-inducible antiviral protein which plays a major role in the cell antiviral state induced by type I and type II interferon. Catalyzes the conversion of cytidine triphosphate (CTP) to 3'-deoxy-3',4'-didehydro-CTP (ddhCTP) via a SAM-dependent radical mechanism. In turn, ddhCTP acts as a chain terminator for the RNA-dependent RNA polymerases from multiple viruses and directly inhibits viral replication. Therefore, inhibits a wide range of DNA and RNA viruses, including human cytomegalovirus (HCMV), hepatitis C virus (HCV), west Nile virus (WNV), dengue virus, sindbis virus, influenza A virus, sendai virus, vesicular stomatitis virus (VSV), zika virus, and human immunodeficiency virus (HIV-1). Also promotes TLR7 and TLR9-dependent production of IFN-beta production in plasmacytoid dendritic cells (pDCs) by facilitating 'Lys-63'-linked ubiquitination of IRAK1 by TRAF6. 1. Yuan, Y. et al. (2020) Molecular cell 77, 734-747.e7. PMID: 31812350 2. Gizzi, AS. et al. (2018) Nature 558, 610-614. PMID: 29925952 3. Dumbrepatil, AB. et al. (2019) The Journal of biological chemistry 294, 6888-6898. PMID: 30872404 4. Wei, C. et al. (2018) Viruses 11. PMID: 30587778 5. Vanwalscappel, B. et al. (2019) International journal of molecular sciences 20. PMID: 30934824 6. Li, M. et al. (2019) Frontiers in immunology 10, 2810. PMID: 31921110 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RSAD2 Protein, N-His
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