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| カタログ番号 | HP499012 |
|---|---|
| 説明 |
Recombinant Human SETX/ALS4/SCAR1 Protein, N-His (HP499012) expressed in E. coli, spanning Ile101-Thr338. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q7Z333 |
| タンパク質長 | Ile101-Thr338 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 30.04 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | SCAR1, ALS4, SETX, SEN1 homolog, Amyotrophic lateral sclerosis 4 protein, KIAA0625, Probable helicase senataxin, Senataxin |
| 背景 | Helicase senataxin (SETX/ALS4) is a ~302 kDa protein. ATP-dependent 5'->3' DNA/RNA helicase that preferentially unwinds RNA substrates over DNA, playing a crucial role in resolving R-loops and promoting transcription termination. Plays a role in transcription regulation by its ability to modulate RNA Polymerase II (Pol II) binding to chromatin and through its interaction with proteins involved in transcription. Contributes to the mRNA splicing efficiency and splice site selection. Required for the resolution of R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site, allowing XRN2 recruitment and XRN2-mediated degradation of the downstream cleaved RNA and hence efficient RNA polymerase II (RNAp II) transcription termination. Required for the 3' transcriptional termination of PER1 and CRY2, thus playing an important role in the circadian rhythm regulation. 1. Hasanova, Z. et al. (2023) Nucleic acids research 51, 2818-2837. PMID: 36864660 2. Suraweera, A. et al. (2009) Human molecular genetics 18, 3384-96. PMID: 19515850 3. Skourti-Stathaki, K. et al. (2011) Molecular cell 42, 794-805. PMID: 21700224 4. Zhao, DY. et al. (2016) Nature 529, 48-53. PMID: 26700805 5. Suraweera, A. et al. (2007) The Journal of cell biology 177, 969-79. PMID: 17562789 6. Richard, P. et al. (2013) Genes & development 27, 2227-32. PMID: 24105744 7. De Amicis, A. et al. (2011) DNA repair 10, 199-209. PMID: 21112256 8. Vantaggiato, C. et al. (2011) Brain : a journal of neurology 134, 1808-28. PMID: 21576111 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SETX/ALS4/SCAR1 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
