Human SF3A3 Recombinant Protein (N-His) (HB387012)

Name: Human SF3A3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB387012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB387012
Description	Recombinant Human SF3A3 Protein, N-His (HB387012) expressed in E. coli, spanning Met1-Leu501. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q12874
Longueur de protéine	Met1-Leu501
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	61.01 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Splicing factor 3A subunit 3; SF3a60; Spliceosome-associated protein 61; SAP 61; SF3A3; SAP61
Contexte	Splicing factor 3A subunit 3 is a ~58 kDa protein. Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs. The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing. Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex. Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes. 1. Das, R. et al. (2000) Molecular cell 5, 779-87. PMID: 10882114 2. Nesic, D. et al. (2001) Molecular and cellular biology 21, 6406-17. PMID: 11533230 3. Zhang, Z. et al. (2020) Nature 583, 310-313. PMID: 32494006 4. Tholen, J. et al. (2022) Science (New York, N.Y.) 375, 50-57. PMID: 34822310 5. Chiara, MD. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 6403-7. PMID: 8022796 6. Zhang, X. et al. (2018) Cell research 28, 307-322. PMID: 29360106 7. Zhan, X. et al. (2018) Cell research 28, 1129-1140. PMID: 30315277
Note	For research use only

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SDS-PAGE for Recombinant Human SF3A3 Protein, N-His

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Human SF3A3 Recombinant Protein (N-His).pdf

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