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| カタログ番号 | HV692012 |
|---|---|
| 説明 |
Recombinant Human SIRT1 Protein, N-His (HV692012) expressed in E. coli, spanning Ile194-Ser747. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96EB6 |
| タンパク質長 | Ile194-Ser747 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 63.02 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | NAD-dependent protein deacylase sirtuin-1, hSIR2, NAD-dependent protein deacetylase sirtuin-1, SIR2-like protein 1, 75SirT1, SIR2L1, Regulatory protein SIR2 homolog 1, SIRT1, hSIRT1 |
| 背景 | NAD-dependent protein deacetylase sirtuin-1 (SIRT1) is a ~81 kDa protein. NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metabolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT). 1. Vaziri, H. et al. (2001) Cell 107, 149-59. PMID: 11672523 2. Langley, E. et al. (2002) The EMBO journal 21, 2383-96. PMID: 12006491 3. Brunet, A. et al. (2004) Science (New York, N.Y.) 303, 2011-5. PMID: 14976264 4. Motta, MC. et al. (2004) Cell 116, 551-63. PMID: 14980222 5. van der Horst, A. et al. (2004) The Journal of biological chemistry 279, 28873-9. PMID: 15126506 6. Yeung, F. et al. (2004) The EMBO journal 23, 2369-80. PMID: 15152190 7. Cohen, HY. et al. (2004) Science (New York, N.Y.) 305, 390-2. PMID: 15205477 8. Vaquero, A. et al. (2004) Molecular cell 16, 93-105. PMID: 15469825 9. Yang, Y. et al. (2005) The EMBO journal 24, 1021-32. PMID: 15692560 10. Michishita, E. et al. (2005) Molecular biology of the cell 16, 4623-35. PMID: 16079181 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SIRT1 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
