

| Каталожный номер | HW629012 |
|---|---|
| Описание |
Recombinant Human SLC1A2 Protein, N-GST (HW629012) expressed in E. coli, spanning Pro460-Lys574. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | P43004 |
| Длина белка | Pro460-Lys574 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 39.77 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | SLC1A2, Glutamate/aspartate transporter II, GLT1, EAAT2, Solute carrier family 1 member 2, Excitatory amino acid transporter 2, Sodium-dependent glutamate/aspartate transporter 2 |
| Фон | Excitatory amino acid transporter 2 (SLC1A2) is a ~62 kDa protein. Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate. Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport. Essential for the rapid removal of released glutamate from the synaptic cleft, and for terminating the postsynaptic action of glutamate. 1. Melzer, N. et al. (2003) The Journal of biological chemistry 278, 50112-9. PMID: 14506254 2. Gendreau, S. et al. (2004) The Journal of biological chemistry 279, 39505-12. PMID: 15265858 3. Abousaab, A. et al. (2016) The Journal of membrane biology 249, 239-49. PMID: 26690923 4. Arriza, JL. et al. (1994) The Journal of neuroscience : the official journal of the Society for Neuroscience 14, 5559-69. PMID: 7521911 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Human SLC1A2 Protein, N-GST
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