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| カタログ番号 | HP316012 |
|---|---|
| 説明 |
Recombinant Human SLC39A4/ZIP4 Protein, N-His (HP316012) expressed in E. coli, spanning Ser23-Tyr327. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6P5W5 |
| タンパク質長 | Ser23-Tyr327 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Solute carrier family 39 member 4, Zinc transporter ZIP4, SLC39A4, Zrt- and Irt-like protein 4, ZIP-4, ZIP4 |
| 背景 | Zinc transporter ZIP4 (SLC39A4) is a ~68 kDa protein. Selective transporter that mediates the uptake of Zn(2+). Plays an essential role for dietary zinc uptake from small intestine. The Zn(2+) uniporter activity is regulated by zinc availability. Also exhibits polyspecific binding and transport of Cu(2+), Cd(2+) and possibly Ni(2+) but at higher concentrations. _x005F_x005F_x000D_ _x005F_x005F_x000D_1. Mao, X. et al. (2007) The Journal of biological chemistry 282, 6992-7000. PMID: 17202136 _x005F_x005F_x000D_
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2. Antala, S. et al. (2012) Biochemistry 51, 963-73. PMID: 22242765 _x005F_x005F_x000D_
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3. Zhang, T. et al. (2016) Nature communications 7, 11979. PMID: 27321477 _x005F_x005F_x000D_
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4. Zhang, T. et al. (2017) Science advances 3, e1700344. PMID: 28875161 _x005F_x005F_x000D_
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5. Zhang, T. et al. (2019) The Biochemical journal 476, 1791-1803. PMID: 31164399 _x005F_x005F_x000D_
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6. Zhang, T. et al. (2020) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34, 237-247. PMID: 31914589 _x005F_x005F_x000D_
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7. Hoch, E. et al. (2020) International journal of molecular sciences 21. PMID: 31979155 _x005F_x005F_x000D_
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8. Kuliyev, E. et al. (2021) The Journal of biological chemistry 296, 100269. PMID: 33837739 _x005F_x005F_x000D_
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9. Liu, Y. et al. (2022) Scientific reports 12, 21083. PMID: 36473915 _x005F_x005F_x000D_ |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SLC39A4/ZIP4 Protein, N-His
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