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| カタログ番号 | HP744012 |
|---|---|
| 説明 |
Recombinant Human SLCO1B1/LST-1/OATP-2 Protein, N-His (HP744012) expressed in E. coli, spanning Phe426-Phe537. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9Y6L6 |
| タンパク質長 | Phe426-Phe537 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 14.72 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | LST1, OATP1B1, OATP2, SLCO1B1, Solute carrier family 21 member 6, OATP-2, Sodium-independent organic anion-transporting polypeptide 2, LST-1, SLC21A6, OATP-C, OATPC, Solute carrier organic anion transporter family member 1B1, Liver-specific organic anion transporter 1 |
| 背景 | Solute carrier organic anion transporter family member 1B1 (SLCO1B1/LST-1) is a ~76 kDa protein. Mediates the Na(+)-independent uptake of organic anions. Shows broad substrate specificity, can transport both organic anions such as bile acid taurocholate (cholyltaurine) and conjugated steroids (dehydroepiandrosterone 3-sulfate, 17-beta-glucuronosyl estradiol, and estrone 3-sulfate), as well as eicosanoids (prostaglandin E2, thromboxane B2, leukotriene C4, and leukotriene E4), and thyroid hormones (T4/L-thyroxine, and T3/3,3',5'-triiodo-L-thyronine). Can take up bilirubin glucuronides from plasma into the liver, contributing to the detoxification-enhancing liver-blood shuttling loop. Involved in the clearance of endogenous and exogenous substrates from the liver. Transports coproporphyrin I and III, by-products of heme synthesis, and may be involved in their hepatic disposition. 1. Abe, T. et al. (1999) The Journal of biological chemistry 274, 17159-63. PMID: 10358072 2. Hirano, M. et al. (2004) The Journal of pharmacology and experimental therapeutics 311, 139-46. PMID: 15159445 3. Mahagita, C. et al. (2007) American journal of physiology. Gastrointestinal and liver physiology 293, G271-8. PMID: 17412826 4. Hsiang, B. et al. (1999) The Journal of biological chemistry 274, 37161-8. PMID: 10601278 5. Tamai, I. et al. (2000) Biochemical and biophysical research communications 273, 251-60. PMID: 10873595 6. Kullak-Ublick, GA. et al. (2001) Gastroenterology 120, 525-33. PMID: 11159893 7. Michalski, C. et al. (2002) The Journal of biological chemistry 277, 43058-63. PMID: 12196548 8. Briz, O. et al. (2003) The Biochemical journal 371, 897-905. PMID: 12568656 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SLCO1B1/LST-1/OATP-2 Protein, N-His
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