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| カタログ番号 | HS868012 |
|---|---|
| 説明 |
Recombinant Human SMN1 Protein, N-His (HS868012) expressed in E. coli, spanning Ala2-Asn294. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q16637 |
| タンパク質長 | Ala2-Asn294 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 34.01 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | SMNC, Survival motor neuron protein, SMNT, Component of gems 1, SMN1, SMN, Gemin-1 |
| 背景 | Survival motor neuron protein (SMN1) is a ~31 kDa protein. The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Within the SMN complex, SMN1 acts as a structural backbone and together with GEMIN2 it gathers the Sm complex subunits. 1. Chari, A. et al. (2008) Cell 135, 497-509. PMID: 18984161 2. Pellizzoni, L. et al. (1998) Cell 95, 615-24. PMID: 9845364 3. Otter, S. et al. (2007) The Journal of biological chemistry 282, 5825-33. PMID: 17178713 4. Zhang, R. et al. (2011) Cell 146, 384-95. PMID: 21816274 5. Tripsianes, K. et al. (2011) Nature structural & molecular biology 18, 1414-20. PMID: 22101937 6. Yi, H. et al. (2020) Nucleic acids research 48, 895-911. PMID: 31799625 7. Lotti, F. et al. (2012) Cell 151, 440-54. PMID: 23063131 8. Zhao, DY. et al. (2016) Nature 529, 48-53. PMID: 26700805 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SMN1 Protein, N-His
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