Human SPTLC2 Recombinant Protein (N-His) (HT093012)

Name: Human SPTLC2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HT093012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HT093012
Description	Recombinant Human SPTLC2 Protein, N-His (HT093012) expressed in E. coli, spanning Gly186-Gln536. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	O15270
Longueur de protéine	Gly186-Gln536
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	40.77 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	LCB2a, SPT 2, Serine palmitoyltransferase 2, LCB 2, KIAA0526, Long chain base biosynthesis protein 2, SPTLC2, LCB2, Long chain base biosynthesis protein 2a, Serine-palmitoyl-CoA transferase 2
Contexte	Serine palmitoyltransferase 2 (SPTLC2) is a ~62 kDa protein. Component of the serine palmitoyltransferase multisubunit enzyme (SPT) that catalyzes the initial and rate-limiting step in sphingolipid biosynthesis by condensing L-serine and activated acyl-CoA (most commonly palmitoyl-CoA) to form long-chain bases. The SPT complex is composed of SPTLC1, SPTLC2 or SPTLC3 and SPTSSA or SPTSSB. Within this complex, the heterodimer consisting of SPTLC1 and SPTLC2/SPTLC3 forms the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates, with a slight preference for C14-CoA. 1. Han, G. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 8186-91. PMID: 19416851 2. Hornemann, T. et al. (2009) The Journal of biological chemistry 284, 26322-30. PMID: 19648650 3. Gable, K. et al. (2010) The Journal of biological chemistry 285, 22846-52. PMID: 20504773 4. Rotthier, A. et al. (2010) American journal of human genetics 87, 513-22. PMID: 20920666
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human SPTLC2 Protein, N-His

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Human SPTLC2 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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