Human SRCAP Recombinant Protein (N-His) (HP108012)

Name: Human SRCAP Recombinant Protein (N-His)
Brand: abinScience
SKU: HP108012
Price: 211 USD
Availability: InStock

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Número de catálogo	HP108012
Descripción	Recombinant Human SRCAP Protein, N-His (HP108012) expressed in E. coli, spanning Lys2044-Thr2197. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	Q6ZRS2
Longitud de proteína	Lys2044-Thr2197
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Helicase SRCAP, 3.6.4.-, Domino homolog 2, Snf2-related CBP activator, SRCAP, KIAA0309
Fondo	Chromatin remodeling protein SRCAP is a ~343 kDa protein. Acts both as a chromatin remodeler and transcription coregulator. Catalytic component of the SRCAP complex which mediates the ATP-dependent exchange of histone H2AZ/H2B dimers for nucleosomal H2A/H2B, leading to transcriptional regulation of selected genes expression through chromatin remodeling. Acts as a coactivator for CREB-mediated transcription, steroid receptor-mediated transcription, and Notch-mediated transcription. _x005F_x005F_x000D_ _x005F_x005F_x000D_ 1. Ruhl, DD. et al. (2006) Biochemistry 45, 5671-7. PMID: 16634648 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 2. Wong, MM. et al. (2007) The Journal of biological chemistry 282, 26132-9. PMID: 17617668 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 3. Johnston, H. et al. (1999) The Journal of biological chemistry 274, 16370-6. PMID: 10347196 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 4. Monroy, MA. et al. (2001) The Journal of biological chemistry 276, 40721-6. PMID: 11522779 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 5. Monroy, MA. et al. (2003) Molecular endocrinology (Baltimore, Md.) 17, 2519-28. PMID: 14500758 _x005F_x005F_x000D_ _x005F_x005F_x000D_ 6. Eissenberg, JC. et al. (2005) Molecular and cellular biology 25, 6559-69. PMID: 16024792 _x005F_x005F_x000D_
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human SRCAP Protein, N-His

Referencias

Datasheet

Human SRCAP Recombinant Protein (N-His).pdf

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Formula

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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